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mus dunni cells  (ATCC)


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    Structured Review

    ATCC mus dunni cells
    Mus Dunni Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 892 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mus+dunni+cells/M%2E+dunni/pm38856651-230-14-17
    Average 96 stars, based on 892 article reviews
    mus dunni cells - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Virus:

    Article Title: Continuous Solvent/Detergent Virus Inactivation Using a Packed-Bed Reactor.
    Article Snippet: For titration, Dulbecco's modified Eagle's medium (DMEM, Gibco, USA) with 4.5 g/L d-glucose, 10% horse serum, 0.5 mM, l-glutamine, 100 mg/L gentamycin sulfate, 2% sodium bicarbonate, 1% NEAS, and 1 mM sodium pyruvate was used. .. Xenotropic murine leukemia virus (X-MuLV) was propagated on Mus dunni cells (ATCC CRL-2017), which were maintained in D-MEM with 10% fetal calf serum (PAA, Austria), 1% L-glutamine, 1% sodium pyruvate, 1% NEAA, and 2% sodium bicarbonate. ..

    Article Title: Evaluation of different RT enzyme standards for quantitation of retroviruses using the single-tube fluorescent product-enhanced reverse transcriptase assay.
    Article Snippet: PCR-based reverse transcriptase (RT) assays are highly sensitive for broad detection of retroviruses.. These assays are currently used for demonstrating the absence of retroviral contamination in vaccines and can also be applied to clinical and laboratory research to investigate low-virus replication.. A single-tube fluorescent product-enhanced reverse transcriptase assay (STF-PERT) has been published that was highly sensitive for retrovirus detection (<10 virions), with enhanced reproducibility and increased efficiency [Sears, J.F., Khan, A.S., 2003.

    Article Title: Truly continuous low pH viral inactivation for biopharmaceutical process integration
    Article Snippet: .. Xenotropic murine leukemia virus (X‐MuLV) was propagated on Mus dunni cells (ATCC CRL‐2017) and titrated on PG4 cells (ATCC CRL‐2032) as described before (Martins et al., ). ..

    Article Title: Functional epitopes on porcine endogenous retrovirus envelope protein interacting with neutralizing antibody combining sites.
    Article Snippet: .. Xenotropic MuLV (ATCC VR1447) was propagated on Mus dunni cells (ATCC CRL-2017) for 14 days to allow the virus to spread completely. ..

    Article Title: Performance of a membrane adsorber for trace impurity removal in biotechnology manufacturing.
    Article Snippet: Membrane adsorbers provide an attractive alternative to traditional bead-based chromatography columns used to remove trace impurities i b D c a fl s ©

    Infection:

    Article Title: Performance of a membrane adsorber for trace impurity removal in biotechnology manufacturing.
    Article Snippet: Membrane adsorbers provide an attractive alternative to traditional bead-based chromatography columns used to remove trace impurities i b D c a fl s ©

    Article Title: Reduced vacuolar ATPase protects mice from Friend virus infection - an unintended but instructive effect in Hif-2afl mice.
    Article Snippet: .. Briefly, titrations (107-102 cells/mL) of single-cell suspensions from infected splenocytes were seeded onto susceptible Mus dunni cells (ATCC #CRL-2017), co-cultivated for 5 days, and stained Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t with F-MuLV envelope-specific monoclonal antibody 720 (Robertson et al. 1991) and horseradish-peroxidase coupled goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) to detect foci. .. Cell-surface staining by flow cytometry Spleen cells were stained as previously described (Schreiber et al. 2017) with fluorochrome-conjugated antibodies and analyzed with a FACSCalibur or FACSCelesta flow cytometer and the FACSDiva software (BD Biosciences, Oxford, UK).

    Single Cell:

    Article Title: Reduced vacuolar ATPase protects mice from Friend virus infection - an unintended but instructive effect in Hif-2afl mice.
    Article Snippet: .. Briefly, titrations (107-102 cells/mL) of single-cell suspensions from infected splenocytes were seeded onto susceptible Mus dunni cells (ATCC #CRL-2017), co-cultivated for 5 days, and stained Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t with F-MuLV envelope-specific monoclonal antibody 720 (Robertson et al. 1991) and horseradish-peroxidase coupled goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) to detect foci. .. Cell-surface staining by flow cytometry Spleen cells were stained as previously described (Schreiber et al. 2017) with fluorochrome-conjugated antibodies and analyzed with a FACSCalibur or FACSCelesta flow cytometer and the FACSDiva software (BD Biosciences, Oxford, UK).

    Staining:

    Article Title: Reduced vacuolar ATPase protects mice from Friend virus infection - an unintended but instructive effect in Hif-2afl mice.
    Article Snippet: .. Briefly, titrations (107-102 cells/mL) of single-cell suspensions from infected splenocytes were seeded onto susceptible Mus dunni cells (ATCC #CRL-2017), co-cultivated for 5 days, and stained Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t with F-MuLV envelope-specific monoclonal antibody 720 (Robertson et al. 1991) and horseradish-peroxidase coupled goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) to detect foci. .. Cell-surface staining by flow cytometry Spleen cells were stained as previously described (Schreiber et al. 2017) with fluorochrome-conjugated antibodies and analyzed with a FACSCalibur or FACSCelesta flow cytometer and the FACSDiva software (BD Biosciences, Oxford, UK).

    Modification:

    Article Title: Parallel evaluation of broad virus detection methods.
    Article Snippet: The testing for adventitious viruses is of critical importance during development and production of biological products.. The recent emergence and ongoing development of broad virus detection methods calls for an evaluation of whether these methods can appropriately be implemented into current adventitious agent testing procedures.. To assess the suitability of several broad virus detection methods, a comparative experimental study was conducted: four virus preparations, which were spiked at two different concentrations each into two different cell culture media, were sent to four investigators in a blinded fashion for analysis with broad virus detection methods such as polymerase chain reaction– electrospray ionization mass spectrometry (PCR-ESI/MS), microarray, and two approaches utilizing massively parallel sequencing.



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    ATCC mus dunni tail fibroblast mdtf cells
    The DD Env confers enhanced utilisation of THTR1 homologues. MLV (FeLV) lacZ pseudotypes bearing the A (Glasgow-1), C (Sarma), DD, ND or NN Envs were plated onto <t>MDTF</t> cells expressing the feline (fe), human (hu) or porcine (po) THTR1 homologues, human FLVCR1 or human FLVCR2. 72 hours post-infection, cells were stained for expression of lacZ and counted manually. Values represent the mean +/− SEM of three independent experiments. The increase in titre associated with the DD mutation upon feline, human or porcine THTR1 is statistically significant in comparison with A (Glasgow-1) (unpaired T test, p < 0.05).
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    Image Search Results


    The DD Env confers enhanced utilisation of THTR1 homologues. MLV (FeLV) lacZ pseudotypes bearing the A (Glasgow-1), C (Sarma), DD, ND or NN Envs were plated onto MDTF cells expressing the feline (fe), human (hu) or porcine (po) THTR1 homologues, human FLVCR1 or human FLVCR2. 72 hours post-infection, cells were stained for expression of lacZ and counted manually. Values represent the mean +/− SEM of three independent experiments. The increase in titre associated with the DD mutation upon feline, human or porcine THTR1 is statistically significant in comparison with A (Glasgow-1) (unpaired T test, p < 0.05).

    Journal: Retrovirology

    Article Title: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

    doi: 10.1186/1742-4690-9-48

    Figure Lengend Snippet: The DD Env confers enhanced utilisation of THTR1 homologues. MLV (FeLV) lacZ pseudotypes bearing the A (Glasgow-1), C (Sarma), DD, ND or NN Envs were plated onto MDTF cells expressing the feline (fe), human (hu) or porcine (po) THTR1 homologues, human FLVCR1 or human FLVCR2. 72 hours post-infection, cells were stained for expression of lacZ and counted manually. Values represent the mean +/− SEM of three independent experiments. The increase in titre associated with the DD mutation upon feline, human or porcine THTR1 is statistically significant in comparison with A (Glasgow-1) (unpaired T test, p < 0.05).

    Article Snippet: Mus dunni tail fibroblast (MDTF) cells (ATCC Catalogue CRL-2017) and guinea pig fibroblast (104 C1) cells were maintained in low-glucose DMEM (Gibco), supplemented with 10% foetal bovine serum, 100U/mL penicillin and 100 μg/mL streptomycin.

    Techniques: Expressing, Infection, Staining, Mutagenesis, Comparison

    The DD mutation confers enhanced binding of Fc-SU to multiple receptors. Matched volumes of supernatant containing the Fc-SU fusion proteins from Glasgow-1 (A), Gardner-Arnstein (B), Sarma (C), or the mutants DD, ND and NN, were added to either MDTF or 104 C1 cells expressing feline, human or porcine THTR1, human FLVCR1 & 2, human Pit1 (MDTF only), or vector only (CON). Fc-SU binding was detected by flow cytometry with PE-conjugated anti-human IgG-Fc. Each histogram represents 10,000 events collected in LIST mode and are representative of two independent analyses. Ordinate displays number of events while abscissa displays fluorescence intensity.

    Journal: Retrovirology

    Article Title: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus

    doi: 10.1186/1742-4690-9-48

    Figure Lengend Snippet: The DD mutation confers enhanced binding of Fc-SU to multiple receptors. Matched volumes of supernatant containing the Fc-SU fusion proteins from Glasgow-1 (A), Gardner-Arnstein (B), Sarma (C), or the mutants DD, ND and NN, were added to either MDTF or 104 C1 cells expressing feline, human or porcine THTR1, human FLVCR1 & 2, human Pit1 (MDTF only), or vector only (CON). Fc-SU binding was detected by flow cytometry with PE-conjugated anti-human IgG-Fc. Each histogram represents 10,000 events collected in LIST mode and are representative of two independent analyses. Ordinate displays number of events while abscissa displays fluorescence intensity.

    Article Snippet: Mus dunni tail fibroblast (MDTF) cells (ATCC Catalogue CRL-2017) and guinea pig fibroblast (104 C1) cells were maintained in low-glucose DMEM (Gibco), supplemented with 10% foetal bovine serum, 100U/mL penicillin and 100 μg/mL streptomycin.

    Techniques: Mutagenesis, Binding Assay, Expressing, Plasmid Preparation, Flow Cytometry, Fluorescence